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Our research presents a link between diminished microglial phagocytosis activity in an Alzheimer mouse model and disruption of purinergic microglial receptor signaling. However, this P2Y6R-mediated potentiation of phagocytic activity is reduced in plaque-associated microglia of older 9 to month-old 5xFAD mice. This impairment of phagocytosis activation by the P2Y6-mediated purinergic signaling pathways is thus not congenital and only develops in later stages of amyloidosis of the 5xFAD Alzheimer mouse model.

Furthermore, we show that uridine diphosphate, a P2Y6 receptor ligand, induces electrophysiological microglia membrane currents that are altered in response and amplitude in microglia in close regional association with plaques, but not in plaque-free regions of 5xFAD animals.

These changes were accompanied by changes in membrane properties and potassium channel activity of plaque-associated microglia in early and late stages of amyloidosis.

Thus, our research results show both a change in the phagocytic activity and the electrophysiological properties of the plaque-associated microglia of the 5xFAD Alzheimer mouse in the context of purinergic signaling. Abstract Perinatal brain damage is a main factor for mortality and long-term morbidity in infants. Several mechanisms have been identified which can lead to perinatal brain injury, such as alterations of oxygen pressure, pharmaceuticals, drugs of abuse, and trauma.

The aim of our study was to identify the pathways and mechanisms leading to brain damage on a molecular und biochemical basis and to analyze the effect of the neuroprotective drug recombinant erythropoietin rEPO. A further aim was to describe the use of the newer and potentially less harmfull antiepileptic drug levetiracteam, which shows little neurotoxicity in experimental animal models and has a good safety records in adults and children.

We found that the treatment with rEPO in rodents showed a reduction in hyperoxia induced apoptosis and lead to an inhibition of the induced proteom changes. These changes were accompanied by reduction in oxidativ stress levels, normalization of raised caspase activity, and reduction of neurotrophins in the brain. In a next step, we investigated acute and long term effects of the administration of dizocilpin NMDA receptor antagonist und phenobarbital GABAA receptor agonist on the developing brain proteome.

Our results highlight various signaling pathways leading to decreased neuronal excitability, particularily when neuronal transduction was altered via NMDA and GABAA receptors.

Since the use of such potentially harmful antikonvulsive drugs is seen critically, we studied the off label use of levetiracetam in term and preterm infants among neonatologists and pediatric neurologists in German university hospitals.

Our results revealed a widespread use of this drug and thus call for randomized and controlled trails investigating efficacy and safety in these cohorts. Plasmodium infection and malaria in school children are increasingly recognized as a relevant public health problem, but data on actual prevalence and health consequences are insufficient.

The present study from highland southern Rwanda aimed at estimating infection prevalence among children attending school, at identifying associated factors and at assessing the clinical consequences of these infections. In a survey including 12 schools in the Huye district of Rwanda, children aged 6—10 years were clinically and anthropometrically examined, malaria parasites were diagnosed by microscopy and PCR, haemoglobin concentrations were measured, and socio- economic and behavioural parameters as well as medical histories were obtained.

Upon examination, the vast majority of children was asymptomatic fever 2. Plasmodium infection was detected in Independent predictors of infection included low altitude, higher age, preceding antimalarial treatment, and absence of electricity or a bicycle in the household.

With the exception of underweight, these conditions were also increased at submicroscopic infection. Malaria infection is frequent among children attending school in southern highland Rwanda. This argues for improved malaria surveillance and control activities among school children. Insgesamt gibt es weltweit nur wenige Studien zu diesem Thema, die zudem uneinheitliche Ergebnisse zeigen. Ein Fragebogen aus insgesamt neun Fragebatterien wurde erstellt.

Zur statistischen Analyse kamen paarweise Tests sowie Clusteranalysen zum Einsatz. Die Teilnehmer waren sich der verschiedenen Therapieoptionen und deren Erfolgsraten bewusst. Obwohl wissenschaftlich belegt, lehnte die Mehrheit der Teilnehmer eine selektive Kariesentfernung ab. While treating deep lesions, the majority of dentists will prefer non-selective complete excavation of demineralized and infected dentin.

Exposure and post-operative complications of the pulp are common complications. Selective incomplete excavation appears to be benefical when treating deep lesions by sealing the caries-affected dentin under a restoration, avoiding pulp exposures and complications. That being said, it is not possible to differentiate between intentionally left, arrested lesions and overlooked or active lesions when viewed radiographically.

This diagnostic uncertainty might pose clinical difficulties and could decrease the acceptance of this concept of therapy. Radiopaque tagging may solve the problem of uncertainty by masking the sealed lesions and assist in discriminating between arrested and progressing lesions.

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